Phenytoin for Wound Healing

Description:

 

Introduction

There are many applications for gel-based topical therapies. There is frequently a desire to apply poorly soluble agents or to apply agents that are not ordinarily soluble at the neutral values of pH required for topical administration. We have applied this to an active ingredient with demonstrated activity in improving wound healing. Phenytoin sodium has well-established use as an anticonvulsant, administered orally or intravenously.  A relatively common side effect is gingival hyperplasia, leading to its proposed use to accelerate wound-healing. Numerous published animal and clinical studies support a positive role for topically administered phenytoin in wound healing including diabetic ulcers, venous ulcers, pressure sores, burns and traumatic wounds. Progress in developing pharmacological treatments to aid in the topical management of wounds has been disappointing and this area remains a huge unmet therapeutic need. The wound care market is estimated to have a value exceeding $10bn annually. While some have attempted to develop cytokine-containing products for application to wounds, results have been disappointing and the active substances are expensive and unstable. The mode of action of phenytoin is likely to be via increased expression of cytokines at a local level.

 

Technology

Investigational studies with phenytoin have often employed the active substance as a powder or as a solution. Often phenytoin was obtained by opening capsules or ampoules. The development of usable formulations is complicated by the poor solubility of phenytoin and the high pH of solutions (around 12), not desirable for application to damaged tissue. Little published work refers to phenytoin in a suitable formulation for convenient and reproducible topical administration. Published prior art in support of the efficacy of phenytoin also provides a potential obstacle to ownership of Intellectual property. The School of Pharmacy at RCSI has developed a product in which phenytoin sodium is incorporated in a matrix that maintains a stable reservoir of phenytoin sodium at a pH in the range 7-8. This reservoir releases phenytoin to the wound in a prolonged fashion. Data in experimental wound models supports the efficacy of the product in wound healing in both diabetic and non-diabetic wounds. Importantly, there is no significant systemic absorption of phenytoin.

 

 

 

 

 

Figure 1: Percentage of control area healed when treated with 1%, 3% and 5% phenytoin gels at three time-points for diabetic rats (n = 8, 10).

 

 

The product is easy to manufacture and scale-up. A 100 kg test batch of a 5% gel has been manufactured and filled with good stability at one year.

 

 

 Figure 2: Stability of a manufactured batch.

 

           Applications

            

Therapies for wound healing address a huge unmet need. In particular the development of pharmacological agents has been slow. There are many opportunities to use or further develop this product for a variety of indications including diabetic ulcers, venous ulcers, pressure sores, burns and traumatic wounds and to incorporate the product into dressings, sprays or adhesive plasters. The increasing prevalence of type 2 diabetes and associated ulcerative conditions makes this one area of significant value.  The technology is also applicable to other active substances such as antiinfectives or analgesics.

 

 Advantages

  • Convenient pharmacological therapy for wounds
  • Applicable to a variety of wounds
  • Good published background
  • Good intellectual Property position
  • Convenient and inexpensive to manufacture
  • Applicable to other active substances

 

 

 

References

  1. Shaw J, Hughes CM, Lagan KM, Bell PM. The clinical effect of topical phenytoin on wound healing: a systematic review.Br J Dermatol. 2007 Nov; 157(5):997-1004.
  2. Arinzon Z, Zeilig G, Berner YN, Adunsky A. Antiepileptic drug use and the occurrence of pressure ulcers among bedridden institutionalized elderly patients: a retrospective chart review. Am J Geriatr Pharmacother. 2005 Sep; 3(3):180-5.

 

 

Contacts:

Dr. Gearóid Tuohy, RCSI Technology Transfer, 123 St Stephen’s Green, Dublin 2, Ireland.

Email: gearoidtuohy@rcsi.ie   Tel: +353 1 4022362

 

 

Phenytoin Formulations and uses thereof in Wound Healing

PCT Date: 03/08/2004

 

Patent Information:
Category(s):
Regenerative Medicine
For Information, Contact:
Gearoid Tuohy
Royal College of Surgeons Ireland
gearoidtuohy@rcsi.ie
Inventors:
John Kelly
Cormac Kennedy
Clare Meaney
Keywords:
Kelly
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